Trucks have blocked highways across Brazil for a second straight day as drivers demand fuel subsidies, better working conditions, and improved road safety.
Local media showed trucks that had been abandoned in the state of Mato Grosso on Wednesday while drivers demonstrated on the Anchieta highway that links Sao Paulo to Brazil's main Santos port.
But the worst hit was the southeastern state of Minas Gerais, where 14 highways were blocked off.
Elsewhere, traffic came to a halt for some six hours after protesters set fire to a bus and vandalized three others on a highway outside the capital Brasilia.
A decision by the authorities to impose fines on those joining the blockade prompted driver union MUBC to ask truckers to move off early Wednesday.
The protesters had blocked off the main access road to Latin America's biggest container port at Santos in the southeast for 24 hours before it re-opened late Tuesday.
The protests come amid the worst social unrest to hit Brazil in more than 20 years, as millions rally against poor public services and rampant corruption.
On Tuesday, riot police fired teargas at truck drivers who had blocked a toll plaza in Sao Paulo while in Belem, riot police clashed with protesters who had stormed City Hall to protest against transport price rises.
Last week, Brazilian President Dilma Rousseff told governors and mayors that her administration would allocate 23 billion US dollars for new spending on urban public transport, without providing details.
Rousseff also met with her cabinet on Monday in order to discuss the ongoing protests that target a wide spectrum of problems, including poor public services and the high cost of hosting next year's World Cup soccer tournament and the 2016 Olympics.
At the height of the protests, the president proposed a referendum for political reform. The announcement caused controversy between Congress, the High Court and the president over the legality of the move.
The opposition accuses Rousseff's Workers' Party of taking advantage of the crisis to make new changes while Supreme Court judges and lawyers say only Congress is legally eligible to pass any changes.